Mutations in exon 11 (11.1 and 11.2) of the <em>BRCA1</em> gene and risk factors for breast cancer in Burkina Faso

Isabelle Touwendpoulimdé  Kiendrebeogo; Abdou Azaque  Zoure; Fabienne Ingrid Zongo; Abdoul Karim  Ouattara; Marie N.L.  Ouedraogo; Jospin  Amegnona; Albert Théophane  Yonli; Bagora Bayala; Nayi Zongo; Aboubacar Hierrhum  Bambara; Alexis Yobi  Sawadogo; Theodora M.  Zohoncon; Dorcas  Obiri-Yeboah; Jacques  Simpore

doi:10.4081/jphia.2022.1921

Authors

Isabelle Touwendpoulimdé Kiendrebeogo Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso. https://orcid.org/0000-0002-4684-4650
Abdou Azaque Zoure Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; Institute of Health Sciences Research, (IRSS/CNRST), Department of Biomedical and Public Health, Ouagadougou, Burkina Faso. https://orcid.org/0000-0002-9423-024X
Fabienne Ingrid Zongo Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou, Burkina Faso.
Abdoul Karim Ouattara
ak.ouattara02@gmail.com
Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso. https://orcid.org/0000-0002-9767-0646
Marie N.L. Ouedraogo Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso.
Jospin Amegnona Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou, Burkina Faso.
Albert Théophane Yonli Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso.
Bagora Bayala Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso.
Nayi Zongo Department of General and Digestive Surgery, University Hospital Center Yalgado Ouedraogo, University Joseph Ki-Zerbo, UFR/SDS, Ouagadougou, Burkina Faso.
Aboubacar Hierrhum Bambara Service of Oncology, University Hospital Center Bogodogo, University Joseph Ki-Zerbo, UFR/SDS, Ouagadougou, Burkina Faso.
Alexis Yobi Sawadogo Service of Gynecology, University Hospital Center Yalgado Ouedraogo, University Joseph Ki-Zerbo, UFR/SDS, Ouagadougou, Burkina Faso.
Theodora M. Zohoncon Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou; Faculty of Health Sciences, University Saint Thomas d’Aquin, Burkina Faso. https://orcid.org/0000-0002-9100-0692
Dorcas Obiri-Yeboah Department of Microbiology and Immunology, School of Medical Sciences, University of Cape Coast, Ghana. https://orcid.org/0000-0003-4562-9294
Jacques Simpore Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou; Faculty of Health Sciences, University Saint Thomas d’Aquin, Burkina Faso. https://orcid.org/0000-0002-0415-9161

Breast cancer is the leading cause of death among women in both developed and developing countries. It is multifactorial, including genetic predispositions such as oncogenic mutations on BRCA1 and 2 genes. The objectives of the present study were to identify oncogenic mutations in exon 11 of the BRCA1 gene and to determine the risk factors for breast cancer among women population in Burkina Faso. This study involved 100 women, including 50 cases of breast cancer and 50 controls (no clinical signs and no family history of breast cancer or other cancers). Mutations in the BRCA1 gene were detected by PCR using sequence primers specific for exon 11 fragments (11.1 and 11.2). In our study population, age (OR=22.40; CI: 4.33-115.82; p<0.001) and obesity (OR=4.23; CI: 1.64-10.92; p=0.003) were risk factors while multiparity was a protective factor for breast cancer (OR=0.35; CI: 0.15-0.81; p=0.02). A mutation was found on both fragments 11.1 and 11.2 of the BRCA1 gene exon 11 in 04/50 (8.0 %) of patients. No mutations were observed in controls. The present study revealed high frequency of oncogenic mutations in exon 11 fragments (11.1 and 11.2) of the BRCA1 gene. These mutations on exon 11 are and involved in the occurrence of breast cancer in our population. Age and obesity were also risk factors for breast cancer among women population in Burkina Faso.

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Kiendrebeogo, I. T. ., Zoure, . A. A. ., Zongo, F. I., Ouattara, A. K. ., Ouedraogo, M. N. ., Amegnona, J. ., Yonli, A. T. ., Bayala, B., Zongo, N., Bambara, A. H. ., Sawadogo, A. Y. ., Zohoncon, T. M. ., Obiri-Yeboah, D. ., & Simpore, J. . (2022). Mutations in exon 11 (11.1 and 11.2) of the <em>BRCA1</em> gene and risk factors for breast cancer in Burkina Faso. Journal of Public Health in Africa, 13(1). https://doi.org/10.4081/jphia.2022.1921