Prevalence of multi-drug resistant <em>Pseudomonas aeruginosa</em> isolated from selected residential sewages in Dutsin-Ma, Katsina State, Nigeria

Authors

  • Ayodele Timilehin Adesoji Department of Microbiology, Federal University Dutsin-Ma, Dutsin-Ma, Katsina State https://orcid.org/0000-0002-8573-952X
  • Jude Prince Onuh Department of Biology and Biochemistry, University of Bath, England https://orcid.org/0000-0003-3985-1470
  • Ishaya Paldung Palang Department of Microbiology, Federal University Dutsin-Ma, Dutsin-Ma, Katsina State
  • Ahmad Mansur Liadi Department of Microbiology, Federal University Dutsin-Ma, Dutsin-Ma, Katsina State https://orcid.org/0000-0003-0185-2646
  • Sule Musa Department of Microbiology, Federal University Dutsin-Ma, Dutsin-Ma, Katsina State

DOI:

https://doi.org/10.4081/jphia.2023.2152

Keywords:

Pseudomonas aeruginosa, residential sewage, antibiotic resistance, Multidrug resistance.

Abstract

The global surge in Multidrug resistant (MDR) bacteria is an issue of great concern. Pseudomonas aeruginosa has been impli- cated in several nosocomial infections, where it has caused grave complications in immunocompromised patients. This is the first study to report the prevalence of MDR P. aeruginosa isolated from residential sewage in Dutsin-Ma, Katsina State, Nigeria. Pseudomonads count, isolation, biochemical characterization and antibiogram were carried out using standard microbiological pro- cedures. This study examined sixty (60) samples from selected res- idential sewage in the study site collected at different intervals between July and September 2021. A total of 40 (66.7%) P. aeruginosa were isolated from the analyzed sewage samples. The high- est (2.84x104) pseudomonad count was recorded from sewage samples collected from Kadangaru. Pseudomonas aeruginosa isolates from this sample site showed the highest (100%) resistance to cephalosporins (cefuroxime) and nitrofurantoin. Similarly, isolates from Miami area also demonstrated the highest (95%) resistance to a cephalosporin (ceftazidime). All (100%) isolates used in this study showed MDR resistance to tested antibiotics. The occurrence of MDR P. aeruginosa from a residential sewage site that may con- taminate drinking water sources in the study area is of public health threat to the inhabitants. Surveillance and molecular epi- demiology of antibiotics resistant bacteria are urgently needed in the study area.

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References

Pier GB, Ramphal R. Pseudomonas aeruginosa. In: Principles and Practise of Infectious Diseases, G. Mandel, J.E. Benneth and R. Dolin, editors. 7th ed. Philadelphia, PA 19103: Churchill Livinstone Elsevier 2010;2835–2860. DOI: https://doi.org/10.1016/B978-0-443-06839-3.00219-8

Frank DW. Pseudomonas aeruginosa, Biology, Genetics, and Host-Pathogen Interactions. Frontiers in Microbiology 2012;1–163. DOI: https://doi.org/10.3389/978-2-88919-016-4

Rami AK, AL-Fridawy WA, Hatite A, Marwa HA. Isolation and Identification of Multidrug Resistance Among Clinical and Environmental Pseudomonas aeruginosa Isolates. Iraqi Journal of Biotechnology 2020;19 (2):37-45.

Purdy-Gibson ME, France M, Hundley TC, Eid N, Remold SK. Pseudomonas aeruginosa in CF and non-CF homes is found predominantly in drains. J Cyst Fibros 2015;14: 341–346. DOI: https://doi.org/10.1016/j.jcf.2014.10.008

Islam A, Babour M, Mohamed AS. Molecular characterization of Pseudomonas aeruginosa isolates from various clinical specimens in Khartoum/Sudan: Antimicrobial resistance and virulence genes. The International Arabic Journal of Antimicrobial Agents 2020;10(1) DOI: https://doi.org/10.3823/840

Tsering DC, Das L, Adhiakari R, Singh T. Extended spectrum beta-lactamase detection in Gram-negative bacilli of nosocomial origin. Journal of Global Infectious Diseases 2009;1(2): 87-92. DOI: https://doi.org/10.4103/0974-777X.56247

Arora D, Jindal N, Kumar R, Romit M. Emerging Antibiotic Resistance in Pseudomonas a Challenge. International Journal of Pharmacy and Pharmaceutical Sciences 2011;3 (2):1488-1491.

Kerr KG, Snelling AM. Pseudomonas aeruginosa: A formidable and ever-present adversary. J Hosp Infect 2009;73:338-344. DOI: https://doi.org/10.1016/j.jhin.2009.04.020

Chika EO, Nneka AR, Dorothy ON, Chika E. Multi-Drug Resistant Pseudomonas aeruginosa Isolated from Hospitals in Onitsha, South-Eastern Nigeria. Int Arch BioMed Clin Res 2017;3(3):17-21. DOI: https://doi.org/10.21276/iabcr.2017.3.3.7

Odoi H, Boamah VE, Boakye YD, Agyare C. Prevalence and Phenotypic and Genotypic Resistance Mechanisms of Multidrug-Resistant Pseudomonas aeruginosa Strains Isolated from Clinical, Environmental, and Poultry Litter Samples from the Ashanti Region of Ghana. J Environ Public Health 2021;9976064. DOI: https://doi.org/10.1155/2021/9976064

Adesoji AT, Abubakar F, Ipinlaye JS. Occurrence of antibiotic resistant bacteria in faeces from abattoir waste, processing water and products from Dutsin-Ma, Katsina State, Nigeria. J Bacteriol Mycol 2016a;3(1):1022.

Rabiu AG, Falodun OI. Multi-drug Resistant Pseudomonas species isolated from the wastewater of an abattoir in Ibadan, Nigeria. J Appl Life Sci Int 2017;13(1):1-9. DOI: https://doi.org/10.9734/JALSI/2017/34522

Igwe PC, Egwu IH, Chukwu SO, Uzoh CV, Oguoma OI, Emeka-Nwabunnia I. Isolation and antibiotics sensitivity pattern of Pseudomonas aeruginosa species implicated with urinary tract infection among women patients visiting Federal teaching Hospital, Abakaliki, Ebonyi State. Direct Res J Publ Hlth Environ Technol 2019;4(4):26-29.

Chika EO, Ejikeugwu PC, Ushie SN, Agbakoba NR. Isolation, Identification and Prevalence of Pseudomonas aeruginosa Isolates from Clinical and Environmental Sources in Onitsha Metropolis, Anambra State. European Journal of Medical and Health Sciences 2020;2(2):1-5.

Odjadjare EO, Ebowemen MJ. Antibiogram of Pseudomonas isolates and potential public health impact of an abattoir effluent in Benin City, Nigeria. African Journal of Clinical and Experimental Microbiology 2020;21:240-249.

Ladan SI. Assessment of Sewage Disposal Methods and Environmental Health Impacts in Katsina Metropolis, Northern Nigeria. Journal of Life Sciences and Technologies. 20142(1):38-43.

Alsaffar M, Jarallah EM. Isolation and Characterization of Lytic Bacteriophages Infecting Pseudomonas aeruginosa From Sewage water. Int. J Pharmtech Res 2016;9: 220-230.

Wikipedia. DutsinMa. Accessed December 6, 2021.

Cheesbrough M. Biochemical tests to identify bacteria. In: District Laboratory Practice in Tropical Countries, 2nd edition. Cambridge University Press, UK. 2006; 178-187. DOI: https://doi.org/10.1017/CBO9780511543470

Clinical and Laboratory Standard Institute (CLSI). Performance standards for antimicrobial susceptibility testing; twenty-fifth informational supplement. In. Wayne, PA 2021; M100-S25. Ezeador CO, Ejikeugwu PC, Ushie SN, Agbakoba NR. Isolation, Identification and Prevalence of Pseudomonas aeruginosa Isolates from Clinical and Environmental Sources in Onitsha Metropolis, Anambra State. European Journal of Medical and Health Sciences. 2020; 2(2). https://doi.org/10.24018/ejmed.2020.2.2.188 DOI: https://doi.org/10.24018/ejmed.2020.2.2.188

Kateete DP, Nakanjako R, Okee M. Genotypic diversity among multidrug resistant Pseudomonas aeruginosa and Acinetobacter species at Mulago Hospital in Kampala, Uganda. BMC Res Notes 2017;10,284. DOI: https://doi.org/10.1186/s13104-017-2612-y

Pletzer D, Braun Y, Weingart H. Swarming motility is modulated by expression of the putative xenosiderophore transporter SppR-SppABCD in Pseudomonas aeruginosa PA14. Antonie Van Leeuwenhoek Van Leeuwenhoek 2016;109:737–753. DOI: https://doi.org/10.1007/s10482-016-0675-8

Adesoji AT, Onuh JP, Okunye OL. Bacteria Resistance to Cephalosporins and its Implication to Public Health. J Bacteriol Mycol 2016b;3(1):1021.

Ejikeugwu C, Duru C, Edeh C, Iroha I. Prevalence of AmpC β-lactamase-producing Pseudomonas aeruginosa Isolates from feacal matter of cow. Journal of Microbiology and Experimentation 2017;4(5):1-4. DOI: https://doi.org/10.15406/jmen.2017.04.00124

Ullah A, Durrani R, Ali G, Ahmed S. Prevalence of antimicrobial resistant Pseudomonas aeruginosa in fresh water spring contaminated with domestic sewage. Journal of Biological and Food Science Research 2012;1(2):19-22.

Ramprasad BP, Rodrigues M, Suprama D. Role of Pseudomonas in nosocomial infections and biological characterization of local strains. Journal of Bioscience Technology 2010;1(14):170–179.

Adesoji AT, Onuh JP, Musa AO, Akinrosoye PF. Bacteriological qualities and antibiogram studies of bacteria from "suya" and smoked fish (Clarias gariepinus) in Dutsin-Ma, Katsina State, Nigeria. Pan African Medical Journal 2019;33;219. DOI: https://doi.org/10.11604/pamj.2019.33.219.17729

Zavascki AP, Carvalhaes C, Picao RC, Gales AC. Multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii: Resistance mechanisms and implications for therapy. Expert Review of Anti-infective Therapy 2010;8(1):71-93. DOI: https://doi.org/10.1586/eri.09.108

Olayinka AT, Olayinka BO, Onile BA. Antibiotic susceptibility and plasmid pattern of Pseudomonas aeruginosa from the surgical unit of a university teaching hospital in north central Nigeria. International Journal of Medicine and Medical Sciences 2009; 1(3):079-083.

Jombo GT, Akpan S, Epoke J, Denen P, Odey AF. Multi-drug resistant Pseudomonas aeruginosa infections complicating. Asian Pacific Journal of Tropical Medicine 2010; 2:479-482. DOI: https://doi.org/10.1016/S1995-7645(10)60115-0

Akingbade OA, Balogun SA, Ojo DA, Afolabi RA, Motayo BO, Okerentugba PO, Okonko IO. Plasmid Profile Analysis of Multidrug Resistant Pseudomonas aeruginosa isolated from wound infections in South West, Nigeria. World Applied Science Journal 2012; 20(6): 766-775.

Igbinosa IH, Tom M, Okoh AI. Antibiogram characteristics and associate Resistance genes of commensal Pseudomonas species isolated from soil and plant rhizosphere in the Eastern Cape Province, South Africa. Journal of Pure and Applied Microbiology 2012;6(4):1541-1551.

Zahra T, Moniri R. Detection of ESBLs and MDR in Pseudomonas aeruginosa in a Tertiary-Care Teaching Hospital. Iranian Journal of Clinical Infectious Diseases 2011; 6(1):18-23.

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Published

01-03-2023

How to Cite

Adesoji, A. T., Onuh, J. P., Palang, I. P., Liadi, A. M., & Musa, S. (2023). Prevalence of multi-drug resistant <em>Pseudomonas aeruginosa</em> isolated from selected residential sewages in Dutsin-Ma, Katsina State, Nigeria. Journal of Public Health in Africa, 14(2). https://doi.org/10.4081/jphia.2023.2152

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