Original Research

In vitro study of pinostrobin propionate and pinostrobin butyrate: Cytotoxic activity against breast cancer cell T47D and its selectivity index

Tri Widiandani, Tiffany Tandian, Bagus D. Zufar, Andi Suryadi, Bambang T. Purwanto, Suko Hardjono, Siswandono Siswandono
Journal of Public Health in Africa | Vol 14, S 1 : 4th Joint Conference of UNAIR-USM, International Conference of Pharmacy and Health Sciences ICPHS| a126 | DOI: https://doi.org/10.4081/jphia.2023.2516 | © 2024 Tri Widiandani, Tiffany Tandian, Bagus D. Zufar, Andi Suryadi, Bambang T. Purwanto, Suko Hardjono, Siswandono Siswandono | This work is licensed under CC Attribution 4.0
Submitted: 03 April 2024 | Published: 30 March 2023

About the author(s)

Tri Widiandani, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia; and, Research Group of Drug Development, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia
Tiffany Tandian, Undergraduate Student of Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia
Bagus D. Zufar, Undergraduate Student of Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia
Andi Suryadi, Department of Pharmacy, Faculty of Sports and Health, Gorontalo State University, Gorontalo, Indonesia
Bambang T. Purwanto, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia; Research Group of Drug Development, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia
Suko Hardjono, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia; Research Group of Drug Development, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia
Siswandono Siswandono, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia; Research Group of Drug Development, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia

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Abstract

Backgrounds: Pinostrobin has the potential activity as an anti-cancer. However, its activity is still lower than the anticancer drugs on the market. To increase its activity, pinostrobin derivatives have been synthesized, namely pinostrobin propionate and pinostrobin butyrate, which are predicted to have better activity and lower toxicity than pinostrobin after being tested by in silico approach. So the compound deserves to be tested for its anticancer activity and selectivity on normal cells. Objective: This study aims to determine the anticancer activity of pinostrobin propionate and pinostrobin butyrate against the T47D breast cancer cell line and its selectivity against the Vero cell line. Methods: The cytotoxicity test which is anticancer activity test and its selectivity on normal cell were carried out using the MTT(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The cells used were breast cancer cell line T47D and normal Vero cells. The test results were analyzed using a microplate reader with a wavelength of 570 nm. Results: From the analysis of anticancer activity on T47D cells, the IC50 values of pinostrobin, pinostrobin propionate, and pinostrobin butyrate were 2.93, 0.57, and 0.40 mM, respectively. While the results of the cytotoxicity test on Vero cells obtained the CC50 value of pinostrobin, pinostrobin propionate, pinostrobin butyrate was 1.27, 0.94, and 0.89 mM, respectively. So the SI value of pinostrobin (SI=0.4) is smaller than its derivatives (SI=1.7 and 2.2). Meanwhile, pinostrobin butyrate is more selective than pinostrobin propionate. Conclusions: It can be concluded that pinostrobin propionate and pinostrobin butyrate compounds have greater activity and selectivity than pinostrobin so these compounds are promising to be further developed as anticancer candidates.

Keywords

pinostrobin propionate; pinostrobin butyrate; breast cancer; cytotoxic activity; selectivity index

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