Original Research

Mutations in exon 11 (11.1 and 11.2) of the BRCA1 gene and risk factors for breast cancer in Burkina Faso

Isabelle T. Kiendrebeogo, Abdou A. Zoure, Fabienne I. Zongo, Abdoul K. Ouattara, Marie N.L. Ouedraogo, Jospin Amegnona, Albert T. Yonli, Bagora Bayala, Nayi Zongo, Aboubacar H. Bambara, Alexis Y. Sawadogo, Theodora M. Zohoncon, Dorcas Obiri-Yeboah, Jacques Simpore
Journal of Public Health in Africa | Vol 13, No 1 | a358 | DOI: https://doi.org/10.4081/jphia.2022.1921 | © 2024 Isabelle T. Kiendrebeogo, Abdou A. Zoure, Fabienne I. Zongo, Abdoul K. Ouattara, Marie N.L. Ouedraogo, Jospin Amegnona, Albert T. Yonli, Bagora Bayala, Nayi Zongo, Aboubacar H. Bambara, Alexis Y. Sawadogo, Theodora M. Zohoncon, Dorcas Obiri-Yeboah, Jacques Simpore | This work is licensed under CC Attribution 4.0
Submitted: 10 April 2024 | Published: 24 May 2022

About the author(s)

Isabelle T. Kiendrebeogo, Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; and, Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Abdou A. Zoure, Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; and, Institute of Health Sciences Research, (IRSS/CNRST), Department of Biomedical and Public Health, Ouagadougou, Burkina Faso
Fabienne I. Zongo, Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
Abdoul K. Ouattara, Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; and, Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Marie N.L. Ouedraogo, Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; and, Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Jospin Amegnona, Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
Albert T. Yonli, Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; and, Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Bagora Bayala, Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; and, Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso
Nayi Zongo, Department of General and Digestive Surgery, University Hospital Center Yalgado Ouedraogo, University Joseph Ki-Zerbo, UFR/SDS, Ouagadougou, Burkina Faso
Aboubacar H. Bambara, Service of Oncology, University Hospital Center Bogodogo, University Joseph Ki-Zerbo, UFR/SDS, Ouagadougou, Burkina Faso
Alexis Y. Sawadogo, Service of Gynecology, University Hospital Center Yalgado Ouedraogo, University Joseph Ki-Zerbo, UFR/SDS, Ouagadougou, Burkina Faso
Theodora M. Zohoncon, Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; and, Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou; and, Faculty of Health Sciences, University Saint Thomas d’Aquin, Burkina Faso
Dorcas Obiri-Yeboah, Department of Microbiology and Immunology, School of Medical Sciences, University of Cape Coast, Ghana
Jacques Simpore, Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR/SVT, University Joseph Ki-Zerbo, Ouagadougou; and, Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou; and, Faculty of Health Sciences, University Saint Thomas d’Aquin, Burkina Faso

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Abstract

Breast cancer is the leading cause of death among women in both developed and developing countries. It is multifactorial, including genetic predispositions such as oncogenic mutations on BRCA1 and 2 genes. The objectives of the present study were to identify oncogenic mutations in exon 11 of the BRCA1 gene and to determine the risk factors for breast cancer among women population in Burkina Faso. This study involved 100 women, including 50 cases of breast cancer and 50 controls (no clinical signs and no family history of breast cancer or other cancers). Mutations in the BRCA1 gene were detected by PCR using sequence primers specific for exon 11 fragments (11.1 and 11.2). In our study population, age (OR=22.40; CI: 4.33-115.82; p<0.001) and obesity (OR=4.23; CI: 1.64-10.92; p=0.003) were risk factors while multiparity was a protective factor for breast cancer (OR=0.35; CI: 0.15-0.81; p=0.02). A mutation was found on both fragments 11.1 and 11.2 of the BRCA1 gene exon 11 in 04/50 (8.0 %) of patients. No mutations were observed in controls. The present study revealed high frequency of oncogenic mutations in exon 11 fragments (11.1 and 11.2) of the BRCA1 gene. These mutations on exon 11 are and involved in the occurrence of breast cancer in our population. Age and obesity were also risk factors for breast cancer among women population in Burkina Faso.

Keywords

BRCA1; Exon 11; cancer; PCR; Mutation; Burkina Faso

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