Original Research

The effect of isolated probiotics from Indonesian Passiflora edulis sims. on interferon gamma levels in peripheral blood mononuclear cell of adult tuberculosis patients in vitro

Iif H. Nurrosyidah, Ni M. Mertaniasih, Isnaeni Isnaeni
Journal of Public Health in Africa | Vol 14, S 1 : 4th Joint Conference of UNAIR-USM, International Conference of Pharmacy and Health Sciences ICPHS| a98 | DOI: https://doi.org/10.4081/jphia.2023.2504 | © 2024 Iif H. Nurrosyidah, Ni M. Mertaniasih, Isnaeni Isnaeni | This work is licensed under CC Attribution 4.0
Submitted: 03 April 2024 | Published: 30 March 2023

About the author(s)

Iif H. Nurrosyidah, Faculty of Health Sciences, Universitas Anwar Medika, Sidoarjo, Indonesia
Ni M. Mertaniasih, Department of Medical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
Isnaeni Isnaeni, Department of Pharmaceutical, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia; and, Faculty of Health Science, Universitas Muhammadiyah Surabaya, Surabaya, Indonesia

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Background: Mycobacterium tuberculosis (M.tb) is unique because the bacteria live intracellularly and hide in macrophages so that they can escape from phagocytosis. This is one of the factors that complicate the treatment of M.tb infections. Interferon-gamma (IFN-γ) is a compound that plays an important role in macrophage activation to control intracellular pathogens. Objective: The purpose of this research is to analyze the concentration of IFN-γ in peripheral blood mononuclear cells of adult Tuberculosis patients (TB) after in vitro administration of multistrain probiotics from indigeneous Indonesian red passion fruit (Passiflora edulis Sims.). Method: The probiotics isolated from red passion fruit were introduced into Peripheral Blood Mononuclear Cell (PBMC) adult TB patients who were undergoing Anti-tuberculosis Drug Therapy (ATD) category one at the Trosobo Primary Health Center (Sidoarjo, East Java, Indonesia), and the patients who were undergoing ATD category one treatment were willing to be involved in this study. Result: The probiotics isolated from fermentation-broth of Indonesian red passion fruit were able to increase IFN-γ levels in PBMC of adult TB patients. Conclusion: The red passion fruit probiotics isolated can increase IFN-γ of adult TB patients increased from 0.82% to 23.17% after in vitro administration.


Interferon gamma, Passiflora edulis Sims, Probiotic, PBMC, Red Passion Fruit, Tuberculosis


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